![]() The lack of a 561-laser means that experiments involving RFP/mCherry should be run on the Astrios instead. Sorting formats: 4-way 5 mL-tube and plate-sorting. The Aria has 3 lasers (407nm, 488nm, 633nm) and is capable of analysing 8 fluorochromes simultaneously. This machine is no longer onsite, so arrangements should be made to run samples on the Astrios instead. ![]() For reagents not on the list, facility staff can help determine compatability. This list is an example of commonly used fluorochromes. * 695 BP 30 is the standard filter and can be swapped for 675 BP 30 to maximise signal from certain fluorochromes Krome Orange, FluoroGold, Pacific Orange, Alexa 430, BV 570, Cascade Yellow, Live/Dead Aqua Pacific Blue, Alexa 405, Creulean, BV421, Horizon V450, DAPI, Hoechst, eFluor 450 *the 488 and 561 lasers share FL1-FL5 detetors.įITC, GFP, CFSE, Alexa 488, Calcein, DyLight 488, Live/Dead GreenĪlexa 546, eFluor 585 NC, dsRed, tdTomatoħ-AAD, PerCP, PerCP-Cy5.5, PE-Cy5, PE-Cy5.5, PerCP-eFluor710ĪPC, Alexa 633, Alexa 647, eFluor 660, Live/Dead Far RedĪPC-Cy7, APC-H7, APC-Alexa750, APC-eFluor780 Pick only one fluorochrome from each row. It is carousel-based, with each carousel holding 32 tubes. The Gallios has 4 lasers (405nm, 488nm, 561nm, 640nm) and is capable of analysing 10 fluorochromes. Software package released by Beckman Coulter for offline analysis Software for analysis of Cytometric Bead Arrays (CBA) from BD Does not have access to additional modules. Older version of FCS express (replaced by v6 in Jan 2017). This licence includes the modules for cell cycle analysis and proliferation analysis. Cytoflex data needs manual adjustment (see cytoflex on the instruments page). Can analyse data from any sorter or analyser. Note: does not have cell cycle or proliferation modules. Can be used for offline analysis of flow data from any analyser or sorter. Please be aware that Detmold Staff may contact you for return of the dongle if your usage has exceeded a fair limit. If the dongle is lost the cost of replacing the software licence will be billed according to its loss.Ī fair use policy applies to all of the software dongles. ![]() Please do not take these dongles off site, i.e. Please do not hold these dongles overnight unless confirmed with a Detmold Staff member that the dongle is not required for installation or training in the morning. ![]()
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